<p>(A) In sham-operated animals, cortical cells displayed diffuse and weak staining for LC3 in the cytosol. After I/R, intense LC3 staining appeared granular in the cytosol of cortical cells. Double staining for LC3 (green) and NeuN (red) showed that increase in LC3 punctate labeling occurred in cortical neurons. (B) In sham-operated animals, cortical cells displayed fine, granular, and perinuclear cathepsin-B staining. After I/R, cathepin-B granules became progressively larger and irregular, and the granular pattern was finally replaced with diffuse cytoplasmic staining. Double staining for cathepsin-B (green) and NeuN (red) showed that increased expression of cathepsin-B occurred mainly in neurons. Bar = 20 µm.</p
