RNase Z is required for <i>milR-4</i> processing.

Abstract

<p>A. A Diagram showing the predicted secondary structure of <i>pri-milR-4</i>. B. RT-PCR analysis, which used a pair of primers indicated in A, showing the presence of transcript that spanning the two alanine tRNAs and <i>milR-4</i>. C. Race tube assays showing the growth rates of the indicated strains in the presence or absence of QA. D. Northern blot analysis showing the levels of <i>rnaseZ</i>-specific siRNA in the indicated strains. E. qRT-PCR analysis showing the reduction of <i>rnaseZ</i> mRNA level in the ds<i>rnaseZ</i> strain in the presence of QA. The asterisk indicates <i>P</i> value<0.05. Error bars indicate S.D. F. Northern blot analysis showing the levels of <i>milR-4</i> milRNAs in the indicated strains in the presence or absence of QA.</p

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