<p>The upper panel shows the construction of URA5-miRs or URA5-miR-ms. Two pair of primers, URA5-XhoI-S/URA5-miRs-BamHI and URA5-BamHI-S/URA5-XbaI-A, were used to PCR amplify the ORF and the terminator regions of <i>URA5</i>, respectively. Among the primers, URA5-miRs-BamHI harbored the sequence of miRs or miR-ms through primer design. The two PCR fragments were then ligated after digested by <i>BamH</i> I. Similarly, the construction of <i>CLC-miR1/2</i> and <i>CLC-miR1-m/miR2-m</i> was made (the bottom part). The position of miRs or miR-ms is indicated by the boxes. Restriction sites are in small letters. Arrows mark the position and direction of the primers. Start codons and stop codons of <i>URA5</i> and <i>CLC1</i> are underlined. For detailed description, see the section of Materials and Methods.</p
