<p>Like in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0050944#pone-0050944-g005" target="_blank">Figure 5</a>, keratinocytes or animals were subjected to UVB treatment and protein extracts from mouse epidermis or from primary keratinocytes cultured in vitro were prepared after 24 h and analyzed by Western blotting. Compared to control keratinocytes, phospho-Akt activation is longer persistent in NRP1-deficient cells (A). Additionally, NRP1-knock out keratinocytes did not activate Rac1 after UVB irradiation (B). All Western blots were repeated independently with extracts from tissue or cells from at least three different animals. The relative expression expresses the fold-change in comparison to the non-irradiated control lane.</p
