Cell characterization and lectin profile of the mouse bone marrow derived macrophages.

Abstract

<p>Histogram showing the percentage of positive stained mouse bone marrow derived macrophages by flow cytometry (open peak-blue line). Isotype control (open peak-black line) and non-stained cells as negative control (shaded peak) of bone marrow derived macrophages stained with (A) CD14 and (B) F4/80. Lectin immune-staining assay to determine the sialic acid (SA) distribution on mouse bone marrow derived macrophages. (C) <i>Maackia amurensis</i> lectin (MAA) conjugated with FITC (the lectin that binds SA-α2,3Gal linked sialic acid) and (D) with <i>Sambucus nigra</i> lectin (SNA) conjugated with FITC (the lectin that binds SA-α2,6GalNAc).</p