<p>Magnifications from SD flat mounted retinas in which FG and Brn3a (<b>A–C</b>); Brn3a detected with two different antibodies -goat and mouse anti-Brn3a-(<b>D–F</b>); Brn3a and b (<b>G–H</b>), Brn3a and c (<b>J–L</b>) and Brn3b and c (<b>M–O</b>) have been double detected. In images like these taken from SD (albino) and PVG (pigmented) rat retinas the percentage of co-localization of each marker (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0049830#pone-0049830-g002" target="_blank">figure 2</a>) was calculated. <b>P–R:</b> cross-sections from SD rat retinas, in which Brn3a (<b>P</b>), Brn3b (<b>Q</b>) and Brn3c (<b>R</b>) have been detected to show that these proteins are only expressed in the ganglion cell layer. In these images, all nuclei have been counterstained with DAPI. Arrows point to those RGCs that only express one of the Brn3s. <i>Bar</i>: 50 µm (C,R).</p

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