Identification of the interaction between RhoC and IQGAP1.

Abstract

<p>(<b>A</b>) BGC-823 cells growing on 100 mm plates were transiently co-infected with Ad-IQGAP1 and Ad-RhoC-V14, or Ad-IQGAP1-C and Ad-RhoC-V14 for 48 h. The cells were lysed and equal amounts of lysate protein were immunoprecipitated (IP) with anti-RhoC, anti-IQGAP1 antibodies or isotype-matched IgG. Whole cell lysate was used as a protein input control. (<b>B</b>) BGC-823 cells were transfected with above adenovirus for 24–48 h, and the co-localization of RhoC and IQGAP1 in cells were determined by Immunofluorescence microscopy using anti-RhoC and anti-IQGAP1 antibodies. Nuclei were stained by Hoechst 33342 (blue). (<b>C</b>) COS-7 cells were transiently co-infected with Ad-IQGAP1-C/Ad-IQGAP1 and Ad-RhoC-V14 for 48 h. The cells were undergoing the same Co-IP procedure described above. (<b>D</b>) COS-7 cells were transfected with above adenoviral vectors for 24–48 h, and the co-localization of RhoC and IQGAP1 in cells were shown by Immunofluorescence. The data are representative from three independent experiments with similar results.</p