The effects of RhoC and IQGAP1 on expression of cell cycle-related proteins.

Abstract

<p>(<b>A</b>) BGC-823 cells were infected with Ad-IQGAP1, Ad-IQGAP1-C or Ad-RhoC-V14 for 48 h, and Western blot was used to analyze the expressions of cyclin E, cyclin D1 cyclin B and CDK. (<b>B</b>) BGC-823 cells were transfected with IQGAP1 siRNA or RhoC siRNA for 72 h, and the expressions of cyclin E, cyclin D1, cyclin B and CDK were analyzed by Western blotting. (<b>C</b>) The protein expressions of cyclin E, cyclin D1, cyclin B and CDK in BGC-823 cells which were transiently transfected with IQGAP1 siRNA or RhoC siRNA for 24 h and afterwards infected with Ad-RhoC-V14, Ad-IQGAP1-C or Ad-IQGAP1 for additional 48 h (Results of Western blotting).</p