The effect of IQGAP1 on proliferation of BGC-823 cells.

Abstract

<p>(<b>A</b>) Western blot analysis of IQGAP1 expression in gastric cancer cell BGC-823 lines infected with Ad-LacZ, Ad-IQGAP1-C, Ad-IQGAP1-N or Ad-IQGAP1. (<b>B</b>) In the MTT assay, IQGAP1-C and IQGAP1 over expression cells both have more proliferation activity than control group (*P<0.05, compared to Ad-LacZ group). (<b>C</b>) The protein expression level of IQGAP1 in gastric cancer cell line BGC-823 transfected with IQGAP1 siRNA. (<b>D</b>) The proliferation of BGC-823 cells transfected with IQGAP1 siRNA were examined by MTT assay. (*P<0.05, compared to Control siRNA group). (<b>E</b>) BGC-823 cells were transiently transfected with plasmids Flag-IQGAP1, Flag-IQGAP1-C, or Flag-IQGAP1-N for 48 h. Western blotting showed the expression of IQGAP1, IQGAP1-N, and IQGAP1-C constructs in BGC-823 cell lines. Equal amounts of cell lysate from each group were loaded and blotted with anti-IQGAP1 antibodies (against C-terminal fragment or N- terminal fragment). (<b>F</b>) BrdU assay was used for analysis cell proliferation. Representative images of BGC-823 cells expressing the indicated IQGAP1 constructs were stained with antibodies against BrdU (second panels red) and Hoechst 33342 for nuclei (first panel, blue). The percentage of cells with BrdU incorporation was calculated. The mean ± SD of three independent experiments is presented (*P<0.05).</p