The proliferation-stimulating effect of RhoC was blocked by IQGAP1 siRNA.

Abstract

<p>(<b>A</b>) The protein expression levels of IQGAP1, IQGAP1-C and RhoC in BGC-823 cells. BGC-823 cells were transiently transfected with IQGAP1 siRNA or RhoC siRNA for 24 h. The transfected cells were afterwards infected with Ad-RhoC-V14, Ad-IQGAP1-C or Ad-IQGAP1 for additional 48 h followed by Western blotting. (<b>B</b>) RhoC depletion did not significantly affect IQGAP1 or IQGAP1-C induced proliferation of BGC-823 cells. (<b>C</b>) The silencing of IQGAP1 by siRNA markedly inhibited the RhoC-induced cell proliferation in BGC-823 cells. (MTT assay, *P<0.05; **P<0.01). The data are the means ± SD from three independent experiments each performed in duplicate.</p