<p>(A), (B), (C), (E), (F), (G). A549 cells were transfected with pCMV5-HA-NS1 and control vector pCMV5, respectively. NS1 was apparent from 24 h post-transfection, mainly in nucleus of A549 cells transfected with pCMV5-HA-NS1 (green color) (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0048340#pone-0048340-g003" target="_blank">Figure 3E</a>). On the other hand, β-tubulin was stained in nucleus and cytoplasm (red color) (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0048340#pone-0048340-g003" target="_blank">Figure 3F</a>).The signals of NS1 and β-tubulin clearly overlapped in nucleus (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0048340#pone-0048340-g003" target="_blank">Figure 3G</a>). (D), (H). The A549 cells transfected with pCMV5-HA-NS1 were stained with Hoechst 33342, and exhibited a stronger blue fluorescence and condensated and fragmented nuclear at 24 h post transfection (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0048340#pone-0048340-g003" target="_blank">Figure 3H</a>).</p
