Compound 2 potentiates GLP-1 9–36 amide-mediated cAMP generation by membranes from HEK-GLP-1R cells.

Abstract

<p>Cell membranes prepared from HEK-GLP-1R cells were incubated with ligands as indicated for 5 min at 30°C in the presence of IBMX before determination of cAMP. A) Concentration-dependent cAMP generation in response to GLP-1 7-36 amide or to GLP-1 9–36 amide either alone or in combination with 3 µM compound 2. <b>B</b>) Responses to GLP-1 9–36 amide or compound 2 alone or the two in combination. The numerical sums of cAMP generation in response to GLP-1 9–36 amide and compound 2 are shown. Data are mean±/+s.e.m., n = 3. For * P<0.05 by Bonferroni's multiple range. For clarity only differences between ‘numerical’ and ‘co-addition’ conditions are shown.</p