<p>Principal coordinates analysis (PCoA) of weighted and unweighted UniFrac distances of IBS patient (green and orange triangles) and healthy control (blue squares and red circles) fecal sample aliquots exposed to room temperature and −80°C for different lengths of time (room temperature - 1, 4, 6, 8 and 24 hours; −80°C −1 week and 1, 2, 3, 4, 5 and 6 months). PCoA plots illustrate the subject each sample aliquot originated from (<b>A&E</b>) and the temperature they were stored at (<b>B&F</b>). Average weighted UniFrac distances for all sample aliquots based on storage at room temperature (<b>C&G</b>) or −80°C (<b>D&H</b>) indicate that sample aliquot microbiotas show significantly similarity (*<i>p</i><0.05).</p

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