Increased LKB1 Serine 307 phosphorylation by Berberine in BAEC.

Abstract

<p><b><i>A. </i></b><b>Inhibition of AMPK activity by ONOO⁻.</b> Recombinant AMPKα1β1γ1 was treated with chemically synthetized ONOO- at concentrations indicated for 5 minutes and AMPK activity was assayed as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0015420#s2" target="_blank">Methods and Materials</a>. N = 5 *P<0.05; control verse ONOO⁻-treated; <i>B</i>. Effects of okadaic acid (OA) on Berberine-enhanced phosphorylation of AMPK-Thr172 and ACC-Ser79 in BAEC. The blot is representative of three blots. from three individual experiments. <i>C</i>. Effects of transfection of PP2A siRNA on Berberine-enhanced phosphorylation of AMPK-Thr172 and ACC-Ser79 in BAEC. The blot is representative of three blots from three individual experiments. <i>D</i>. Effects of STO-609 on Berberine-enhanced phosphorylation of AMPK-Thr172 and ACC-Ser79 in BAEC. The blot is representative of three blots. <i>E.</i> Effects of berberine on the phosphorylation of LKB1 serine 307 in BAEC. The blot is representative of three blots from three individual experiments. <i>F</i>. Genetic inhibition of LKB1 abolishes Berberine-induced phosphorylation of AMPK-Thr172 in BAEC. The blot is representative of three blots from three individual experiments. <i>G</i> Effects of Berberine on AMPK phosphorylation at Thr172 in LKB1-deficient A549 cells.</p