<p>PBMCs obtained before ASFV challenge from pigs immunized with pCMV, pCMV-PQ or pCMV-sHAPQ (immunized three or four times), were <i>in vitro</i> stimulated with media (negative control), with the E75 ASFV isolate (10<sup>5</sup> HAU<sub>50</sub>/ml), with a mix of the p54, p30 and HA ASFV proteins (6 μg/ml of each one) or with each one of these proteins individually. Values shown correspond to the average number of IFNγ-secretory cells detected per million of PBMCs. Standard deviations found within each group are also represented.</p