Alignment of genomic sequences spanning exon X of <i>C. similis</i>-T2L amelogenin gene with the selected species.

Abstract

<p>The black spiny-tailed iguana genomic sequence spanning the exon X was amplified by PCR and analyzed by alignment with the Carolina anole in the same region of the black spiny-tailed iguana exon X sequence. Like the majority of exons, a conserved sequence feature, the presence of AG at the 5′ splice site and GT at the 3′ splice site were observed spanning the exon X, while the same sequence region of the Carolina anole share a high sequence identity except the lack of G in AG at the 5′ splice site, which fail to generate a exon X observed in the black spiny-tailed iguana. The 5′ and 3′ splice sites in the black spiny-tailed iguana genomic sequence spanning the exon X are highlighted as red. Alignment gaps are indicated by a dash (−).</p