Induction of CD4+CD25+Foxp3+Tregs by MDS-MSC is dependent on TGFβ1.

Abstract

<p>Western blot confirmed efficient knockdown of TGF-β1. (B) CD4+CD25-T cells were cultured with TGF-β1 knockdown MDS-MSC or normal-MSC for 5 days, and CD4+ T cells were collected. The expression of CD25 and Foxp3 on CD4+ T cells was analyzed by FACS. Results are expressed as mean±SD of triplicates of 5 separate experiments. *P≤0.05. (C) CD4+CD25-T cells were cultured with mutant siRNA transfected MDS-MSC or normal-MSC for 5 days, and CD4+ T cells were collected. The expression of CD25 and Foxp3 on CD4+ T cells was analyzed by FACS. Results are expressed as mean±SD of triplicates of 6 separate experiments. *P≤0.05. (D) anti-rhTGF-β1 mAb was added at the beginning of coculture of CD4+CD25-T cells and untransfected MDS-MSC or normal-MSC for 5 days, and CD4+ T cells were collected. The expression of CD25 and Foxp3 on CD4+ T cells was analyzed by FACS. Results are expressed as mean±SD of triplicates of 6 separate experiments. *P≤0.05.</p

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