Abstract

<p>AOX mRNA was determined by real time PCR as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0044139#s4" target="_blank">Material and Methods</a> (A). Results represent the AOX/β actin ratio of duplicate experimental determination of 6 different biological samples of female control (FC) and Cd-treated offspring (FT) and male control (MC) and Cd-treated offspring (MT). Western blot analysis for AOX protein expression in liver of control (FC) and treated female (FT) and control (MC) and treated male (MT) fetus (B). AOX western blot signals were determined by densitometry and expressed as abundance ratios of AOX related to actin (C); *p<0.05 compared with respective control.</p

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