Plasmid DNA and morpholinos were efficiently introduced into mouse preimplantation embryos by electroporation.

Abstract

<p>1-cell embryos, 2-cell embryos, 4-cell embryos, 8-cell embryos and morulas were electroporated with pIRES2-AcGFP1-Nuc (A) or the lissamine conjugated morpholinos (B) and cultured for 24 h. pIRES2-AcGFP1-Nuc and morpholinos introduce, revealed by green fluorescent protein and lissamine fluorescence, respectively, is efficient. 4-cell embryos and 8-cell embryos were all developed from the electroporated 2-cell embryos. Original magnification was ×200.</p