NO addition attenuates outward current of two subsets of PNs <i>in vitro</i>.

Abstract

<p><b>A</b>. Representative trace of PN with basal outward current <i>in vitro</i> showing (left to right) saline (control), ProliNO (100 mM) and subtracted current responses, elicited by the depolarization protocol (<i>bottom</i>): Voltage stepped from −90 to +50 mV in 10 mV increments for 100 ms from a holding potential of −70 mV. <b>B</b>. I–V plot of normalized current for control, increasing ProliNO doses (1, 10, 100, &250 mM), and washout (mean, n = 4). <b>C</b>. NO addition inhibited significantly the steady-state outward current of PNs with basal outward current in a dose-dependent manner (means +/− SD, n = 4). <b>D–F</b>. NO addition enhances significantly inward current responses of PNs with basal inward current. <b>D</b>. Representative traces of saline, ProliNO (100 mM) and subtracted current responses. <b>E, F</b>. Normalized I–V plot and histogram showing responses of <i>in vitro</i> PNs with basal inward current responses to increasing doses of ProliNO (Means +/− SD, n = 4).</p