<p><b>a</b>, Western blotting analysis confirms down-regulation of E-cadherin and up-regulation of N-cadherin during EMT. <b>b</b>,<b>c</b>, Western blotting of E-cadherin and N-cadherin in HK-2 cells treated with TGF-beta and transfected with control miR or miR-200 members precursors individually. In western blotting, each band has been quantitated and subjected to densitometry to determine if statistically significant difference exists between groups <b>d</b>, Changes in miR-200 family levels in UUO model mice kidneys, as measured by <i>TaqMan</i> qRT-PCR and normalized to U6 expression. The data are means from a representative time course experiment measured in triplicate and are presented as mean±S.E. <b>e</b>, Azan staining of intact Balbc mouse kidney, UUO day-6 kidneys after transfection of negative control pre-miR or miR-200b precursor. Fibrosis score; The tubulointerstitial fibrosis score in kidneys of miR-200b precursor injected group was significantly lower than of control group: <i>P</i><0.05, The data are means from experiment measured in triplicate and are presented as mean±S.E (n = 8) <b>f</b>, Immunofluorescence studies of vimentin and cytokeratin 18 in the kidneys of UUO day6 mice and UUO day6 mice injected by miR-200b precursor. miR-200b ameliorates up-regulation of vimentin and down-regulation of cytokeratin 18. <b>g</b>, real-time PCR confirms that miR-200b precursor reverses up-regulation of type I,III collagen and fibronectin of UUO day-6 kidneys. The data are normalized to beta-actin mRNA and shown as mean±S.E.(n = 8).</p
