Inhibition of influenza infection and replication by RB19 in MDCK cells.

Abstract

<p>(A) RB19 inhibits the influenza-induced cytopathic effect. In the antiviral neutralization test, MDCK cells were lysed 64 h after A/WSN/33 infection, as shown in the VC (virus control) column. The agent RB19 was added to A/WSN/33-infected cells by two-fold serial dilution starting with a concentration of 50 µM (leftmost column). (B) Reduction in viral yields from infected cells treat w/o RB19 at different concentrations. MDCK cells were infected with MOI 0.001 A/WSN/33 (H1N1) and various concentrations of RB19 were added at the adsorption stage of the A/WSN/33 replication cycle. At 48 h post infection, culture supernatants were collected for virus titration using neuraminidase activity to monitor the viral yield. (C) Inhibition of influenza virus plaque formation by RB19. Approximately 50–100 PFU/well of A/WSN/33 (H1N1) or A/Udorn/72 (H3N2) of influenza A virus was used to infect MDCK cells in 6-well plates. After the viral adsorption stage, 3 ml of agar was overlayed on the media containing various concentrations of RB19. The concentration of RB19 is indicated at the top.</p