Comparative morphology and phenotype between THP-1, N₂ and Nef upon co-stimulation with GM-CSF,IL-4,TNFα and ionomycin.

Abstract

<p>(A): morphology of the above cell types was observed in light background (upper panel) and black one (lower panel) at magnification of 400X; (B): The histograms showed a direct comparison of CD11c, CD14, CD40,CD80,CD83,CD86,HLA-DR levels for Nef <i>vs</i> N₂ and THP-1,(7-day culture based on “5+2” protocol ); (C): The histograms showed a direct comparison of above surface markers expression levels for Nef <i>vs</i> N₂ and THP-1 (2-day culture based on “0+2” protocol). One representative of three performed experiments was presented.</p