<p>(Panel A) Diagram of Czf1p. Numbers indicate amino acid residues. Gln, glutamine rich region. ZnF, zinc finger motif. (Panel B) Alignment of the zinc-finger motif amino acid sequence of Czf1p (residues 315–342) and <i>S. cerevisiae</i> Gal4p (residues 11–38). Marked regions A and B, and consensus sequence are from Corton et al. (1998) and are based on amino acids that are conserved in more than 33% of sequences from 79 Cys<sub>6</sub>Zn<sub>2</sub> fungal proteins. Lys17 and Lys18 in Gal4p make direct contacts with the target DNA sequence. Underlined residues in the Czf1p amino acid sequence were targeted in this study.</p