<p>(A, C, D) The sensitivity of A-375 and A-375-TS cells for TRAM/TRAIL-induced apoptosis is shown (A) after siRNA-mediated Bid knockdown, (C) plasmid-mediated XIAP overexpression and (D) siRNA-mediated SMAC knockdown. The respective mock controls are shown for comparison. Apoptosis (% of sub-G1 cells) and cytotoxicity (LDH release) is shown at 24 h of treatment. Mean values and SDs of three (XIAP, SMAC) or two independent experiments (Bid) are shown; each experiment consisted of triplicates. Statistical significance is indicated (*; p<0.005), when comparing to the respective mock controls. Overexpression or downregulation is shown in insets. (B) Expression of cIAPs (survivin, XIAP and cIAP-2) as well as of SMAC was analyzed by Western blotting in total protein extracts of melanoma cell lines.</p
