<p>(A) Mitochondrial extracts of A-375, treated as indicated with TRAM-34 and TRAIL for 24 h, were analyzed for IK1 by Western blotting. Comparable amounts of mitochondrial extracts were loaded as proven by incubation with prohibitin antibody. A cytosolic extract (Cyto) as well as total protein extracts of mock or IK1-transfected HEK-293 served as controls. (B, C) Mitochondrial (Mito) and cytosolic extracts (Cyto) of A-375 and A-375-TS treated for 4 h with TRAM-34 (40 µM) +/−TRAIL were investigated by Western blotting. Equal loading was proven by the mitochondrial protein VDAC and cytosolic protein GAPDH, respectively. VDAC applied to cytosolic extracts ruled out contaminations with mitochondria.</p
