Primary cilium projections from the surface of an MC3T3-E1 osteoblast and its bending during radiation of pulsed ultrasound.

Abstract

<p>The upper panel shows primary cilium stained with an antibody against acetylated α-tubulin (red) (arrowheads, A and D), cell body stained with CMFDA (green) (B and D) and nucleus stained with DAPI (blue) (C and D) in a MC3T3-E1 osteoblast. The lower panel shows the top views of bending movement of a primary cilium (arrowheads) extending from the surface of an MC3T3-E1 osteoblast before (E) and during (F and G) application of pulsed ultrasound from top to bottom. Scale bar, 10 µm.</p