<p>(A, C, E) Immunohistochemical assays of the expression of PCNA (A), Col I (C) and OCN (E). (B, D, F) Bar graphs represent their expression density in unit area of bone marrow or unit area of bone trabeculae. (A) OBs; (C) a. OBs, b. osteocytes, c. trabeculae, d. VECs, e. OCs. The expression of PCNA decreased after trauma, and began to be detected in OBs 2 weeks after trauma and was maintained during the following time. Col I increased in the bone marrow for less than 1 week after trauma, and was significantly accumulated in the trabeculae by the end of the study. Both OBs and OCs also expressed increased levels of Col I following trauma. A significant decrease in OCN was detected 3 days after trauma, but it increased significantly 2 weeks later. High levels of OCN were consistently associated with peri-VECs and the fibrous medulla. N: normal group; 3 d, 1 w, 2 w, 3 w: 3 days, 1 week, 1 weeks, 3 weeks post-trauma, respectively. Quantification was based on at least 10 fields per section. *<i>P</i><0.05 vs. normal group.</p
