<p>(A) Unequal distribution of the blood vasculature (red) of a whole HT29 tumour vibratome slice (stitched of ×10 objective lens images). (B) Magnification of the boxed area in A, showing immature blood vessels (projection of a z series with z step size of 2.85 µm over 215 µm with ×10 objective lens). (C) Three-dimensional view of a section of B (z series with z step size of 1.4 µm over 140 µm with ×40 objective lens) showing the irregular structure of blood vessels. All tissues were viewed by confocal fluorescence microscopy (Nikon A1R confocal microscope with plan apo ×10/0.45 numerical aperture (NA) or plan fluo oil ×40/1.3 NA objective lens and with the laser excitation wavelength of 561 nm and the emission of 595). Scale bars: 2000 µm for A, 200 µm for B.</p
