<p>AMPK suppression in HUVEC either by (A) compound C (10 µmol/L for 2 hour) or by (B) overexpression of AMPK-dominant negative mutant (DN) decreases both the OGT association with proteasome and the O-GlcNAcylation of PA700/S10B (vs. controls), which can be reversed by AICAR pre-incubation (2 mmol/L for 6 hours) in AMPK-present but not AMPK-DN cell. In contrast, constitutive activation of AMPK (overexpression of AMPK-CA vs GFP) (B) increases OGT-PA700 association. All of the blots shown are representative of 3 independent experiments. NS reprents not significant.</p
