<p>Compound C (10 µmol/L for 2 hour)-treated HUVEC present (A) AMPK inactivation, (B) an increase in association of PA700/S10B (from 19S complex) with β7 (from 20S complex), which can be blocked by AICAR pre-incubation (2 mmol/L for 6 hours), (C) 26S proteasome assembly (same samples were run on 3–14% native-PAGE under non-reducing condition followed by conventional Western blot on duplicated blots with PA700/S10B and β7 antibodies, respectively), and (D) an increase in 26S proteasome activity (chymotrypsin-like) (n = 3). The increased association of proteasome sub-complex is also observed in (E) HUVEC overexpressing AMPK-DN but not AMPK-CA or GFP. All of the blots shown are representative of 3 independent experiments. NS represents not significant.</p
