Stimulation of p21 expression by JMJD2D in U2OS cells.

Abstract

<p>(<b>A</b>) Wild-type or H192A JMJD2D or empty vector pEV3S were stably transfected into U2OS cells. Expression of indicated proteins was assessed by Western blotting in cells treated for 24 h with 1 µM adriamycin or DMSO as a control. (<b>B</b>) RNA was isolated from stably transfected U2OS cells and RT-PCR analyses were performed. Shown is the amplification of p21 cDNA and, as a control, GAPDH cDNA.</p