Expression of selected genes of cellular processes by microarray and qPCR in membrane cultured IPEC-J2 cells in response to apical and basolateral DON application.

Abstract

<p>Regulation of transcripts of (<b>A</b>) LAMP2 (lysosomes), (<b>B</b>) CDKN1A (cell cycle), (<b>C</b>) CTNNB1 (focal adhesion) and (<b>D</b>) CLDN3 (tight junction) in response to apical and basolateral DON (200 and 2000 ng/mL) treatment. The mRNA levels are given as fold increase over untreated control. Bars represent means of 3 (microarray) and 5 (qPCR) independent experiments (±SEM). Significant differences to untreated control were calculated by ANOVA and Dunnett's post test (* p≤0.05; ** p≤0.01).</p

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