Activation of the p21 promoter by JMJD2D in HEK293T cells.

Abstract

<p>(<b>A</b>) Activity of a p21 luciferase reporter construct upon cotransfection of control vector pEV3S or wild-type or H192A JMJD2D is depicted. As indicated, empty vector pcDNA3 or pcDNA3-p53 was also transfected. (<b>B</b>) Analogous, response of a CMV or MMP-1 luciferase reporter construct to cotransfection of p53 and/or JMJD2D. (<b>C</b>) Chromatin immunoprecipitation assay in HCT116 cells treated without and with adriamycin.</p