Multiple genes expression and rotavirus-VLPs production in silkworm.

Abstract

<p>(a) The larvae of six days post injection were observed using the fluorescence detection device. 1: the mock injected larvae; 2&3: the larvae injected with invasive and DAP auxotrophic <i>E. coli</i> carrying BmBacmid with six genes including <i>egfp</i>, <i>dsRed</i>, <i>eyfp</i>, <i>vp2</i>, <i>vp6</i> and <i>vp7</i> at a dose of 8.0×10⁸ cells per larva. (b) The hemolymph from a red larva was observed using laser confocal microscope. The images of hemocytes were taken at the bright (trans) channel (I), GFP (515 nm) detection channel (II), DsRed (590 nm) channel (III) and YFP (530 nm) channel (IV). (c) Western blot analysis of the hemolymph from the red larvae using anti-VP2, anti-VP6 and anti-VP7 rabbit antiserums. (d) The EM image of hemocytes collected from red larvae. R: the round Rotavirus-VLPs; B: the rod shape baculovirus particle, bar = 100 nm.</p