<i>In vitro</i> MLV-LTR integration into <i>Stat5a</i>.

Abstract

<p>The vertical axis to the left represents the number of integrations into each nucleotide in M0 (native <i>Stat5a</i>), modified sequences M1-M4, and control same length random sequences R1-R5. These sequences are 400-bp in length, portions of which are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031533#pone-0031533-g001" target="_blank">Figure 1</a>. The horizontal axis represents the bases 1105–1153 in the <i>Stat5a</i> gene. The sequences shown are the junction of the target sequence and 5′- MLV LTR when the MLV is inserted at nucleotide 1130. (A) Integration sites identified with the <i>in vitro</i> assay using sequences M0-M4. Black circles (L) represent the number of mice suffering from lymphomas resulting from MLV integration into the individual nucleotides shown. The number of integrations into nucleotide 1130 per 2000 integrations was significantly greater with sequences M0 and M1 than with sequences M2-M5 (*<i>P</i><0.05). (B) Integration sites identified with the <i>in vitro</i> assay using the 5 random sequences (R1-R5) inserted into the plasmid DNA.</p