Locus of the <i>gsb</i> gene.

Abstract

<p>(<b>A</b>) <i>gsb</i> mutant alleles. The two deficiencies, <i>Df(2R)IIX62</i> and <i>Df(2R)SB1</i>, as well as the two hypomorphic alleles, <i>gsb525</i> and <i>gsbP1155</i>, are depicted. Neighboring genes uncovered by <i>Df(2R)IIX62</i>, <i>zip</i>, <i>uzip</i>, <i>CG3441</i>, and <i>gsbn</i> upstream of <i>gsb</i>, <i>gol</i> and <i>dTKR</i> downstream of <i>gsb</i>, and their direction of transcription are indicated (the rigth telomere of the second chromosome is to the right). Exons are marked by black boxes in the enlarged portion of (<b>A</b>) and also in (<b>B</b>). (<b>B</b>) Map of <i>gsb0-</i>525 abd <i>gsb0</i>-ΔHC transgenes. Both transgenes contain the upstream epidermis enhancers of <i>gsb</i>, GEE and GLE (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030980#pone-0030980-g001" target="_blank"><b>Fig. 1A</b></a>; Li et al., 1993), the <i>gsb</i> promoter, and the entire 3′ UTR of <i>gsb</i>. In <i>gsb0</i>-ΔHC, 519 bp of coding region between the <i>gsb525</i> mutation and a <i>SacII</i> site are deleted, resulting in a shift of the open reading frame after the <i>gsb525</i> nonsense mutation. (<b>C</b>) Sequence surrounding the <i>gsbP1155</i> insertion site. The negative numbers refer to nucleotides upstream of the transcription start site. The eight nucleotides, duplicated during insertion of the P-element, are underlined.</p