Map of <i>gsb-res</i>, <i>gsb-</i>Prd and <i>gsb-</i>Pax3 transgenes.

Abstract

<p>The <i>gsb-res</i> transgene corresponds to the enlarged 20-kb genomic fragment in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030980#pone-0030980-g001" target="_blank"><b>Fig. 1A</b></a>, which includes the <i>gsb</i> transcribed region as well as adjacent 14-kb upstream and 3-kb downstream sequences <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030980#pone.0030980-Gutjahr1" target="_blank">[9]</a>. The upstream sequence also contains the 5′ portion of the <i>gsbn</i> up to part of the third exon. In <i>gsb</i>-Prd and <i>gsb</i>-Pax3 transgenes, the <i>gsb</i> coding region (except of a small region encoding the C-terminus) is replaced by <i>prd</i> and <i>Pax3</i> cDNAs, while upstream and downstream regions are retained. The <i>gsb</i> intron is also retained by inserting it between sequences of the <i>gsb</i> and <i>prd</i> or <i>Pax3</i> leaders. Coding regions are indicated as black boxes except for the paired-domain (PD) and the <i>prd</i>-type homeodomain (HD) which are hatched. The <i>gsb</i> and <i>gsbn</i> introns are indicated as open boxes. The transcription start of <i>gsb</i> is marked by 0, and poly(A) addition signals AATAAA are indicated.</p