Rescue of the CNS phenotype of <i>gsb</i> mutant embryos by <i>gsb</i>-Prd and <i>gsb</i>-Pax3 transgenes.

Abstract

<p>Patterns of longitudinal and commissural axons in the CNS of wild-type (<i>ry⁵⁰⁶</i>; <b>A</b>) and <i>Df(2R)IIX62/gsb525</i> embryos without (<b>B</b>) and with one copy of the <i>gsb</i>-Prd (<b>C</b>) or <i>gsb</i>-Pax3 transgene (<b>D</b>). Embryos at stage 15 were collected from crosses between <i>Df(2R)IIX62/CyO</i>, <i>hb-</i>LacZ; <i>gsb</i>-Prd/+ or <i>Df(2R)IIX62/CyO</i>, <i>hb-</i>LacZ; <i>gsb-</i>Pax3/+ males and <i>gsb525/CyO</i>, <i>hb-</i>LacZ females, and double stained with rabbit antiserum against ß-galactosidase and monoclonal antibody BP102. One quarter of the embryos did not stain for ß-galactosidase as expected. Half of these embryos have missing or reduced posterior commissures as expected for <i>gsb</i> mutants, the other half displays fully rescued commissural patterns as in wild-type embryos. Scale bar: 10 um.</p