<p>A) Alignment of regions S and W, the proposed DimB binding sites in region A of the <i>pspA</i> promoter, with the known DimB binding sites within the <i>ecmA</i> promoter: R2 and R1. Also indicated, above the sequence, are the positions of the point mutations used in scanning analysis of DimB binding. B) Total nuclear extracts obtained from Ax-2 and dimB- slug cells used in gel retardation with a region A probe. The competitors are the R2 and R2M sequences from within the <i>ecmA</i> promoter <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0029895#pone.0029895-Zhukovskaya1" target="_blank">[8]</a> C) Total nuclear extracts obtained from Ax-2 slug cells used in gel retardation with a region A probe. The competitors are region A itself and scanning mutants M1 to M6. D) Gel retardation with recombinant DimB using an A region probe. Competitors are: A itself, and oligonucleotide M145, containing region A with mutations M1, M4 and M5 that collectively mutate the S and W DimB binding sites. Again, the control competitors are the R2 and R2M sequences from within the <i>ecmA</i> promoter.</p
