Cellularization microfilament defects in <i>src64</i> mutant embryos.

Abstract

<p>Embryos are stained with antibody to myosin II heavy chain. (A) Cross-sections showing the early cellularization front. Furrow canal depths are less uniform, producing a wavy and irregular cellularization front in <i>src64^Δ17</i>, <i>src64^H402L</i>, <i>src64^D404N</i> and <i>src64^KO</i> mutant embryos. Scale bar, 50 µm. (B) Grazing section projections of early cellularization stage embryos showing the microfilament network. Microfilament rings of <i>src64^Δ17</i>, <i>src64^H402L</i>, <i>src64^D404N</i> and <i>src64^KO</i> mutant embryos are irregular. Scale bar, 10 µm. (C) Grazing section projections of late cellularization stage embryos showing microfilament rings. Microfilament rings of <i>src64^Δ17</i>, <i>src64^H402L</i>, <i>src64^D404N</i> and <i>src64^KO</i> mutant embryos are irregular and less constricted than the microfilament rings of wild-type embryos and <i>src64^R403C</i> mutant embryos. Scale bar, 10 µm. (D) Microfilament ring circularity during early cellularization. A, does not differ from <i>+</i>; AB, differs from <i>+</i> and <i>src64^Δ17</i>; B, does not differ from <i>src64^Δ17</i> allele but differs from <i>+</i> (P<0.01); C, does not differ from <i>src64^KO</i> but differs from <i>+</i> (P<0.001) and <i>src64^Δ17</i> (P<0.001). Error bars are SEM. (E) Microfilament ring circularity during late cellularization. A, does not differ from <i>+</i>; B, does not differ from <i>src64^Δ17</i> but differs from <i>+</i> (P<0.05); C, does not differ from <i>src64^KO</i> but differs from <i>+</i> (P<0.001) and <i>src64^Δ17</i> (P<0.001). Error bars are SEM.</p