<p>(A–E) HUVEC pretreated for 30 min with 100 nM 16K hPRL (16K) were cultured for 6 h in the presence or absence of HBVP. The two cell populations were then separated prior to qRT-PCR analysis and subjected to qRT-PCR analysis to detect <i>DLL4</i>, <i>NOTCH4</i>, <i>NOTCH1</i>, <i>HEY2</i> and <i>EPHRINB2</i> transcripts. (F) Western blot analysis to detect Notch1 ICD. Data are presented as means ± SEM, n = 3 and are representative of at least two different experiments. n.s., non significant. *, P<0.05; ***, P<0.0001.</p
