<p>In cultures of HBVP or HUVEC alone, the cells were treated with 100 nM 16K hPRL (16K) for 16 h. HBVP+HUVEC cocultures were seeded and treated as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0027318#pone-0027318-g006" target="_blank">Fig. 6</a>, but the time spent in coculture was longer (16 h). The two cell populations were then separated prior to qRT-PCR analysis. In HBVP, transcripts of <i>NOTCH3</i> and <i>aSMA</i> were detected (respectively A and C). Western blot analysis was also performed to detect Notch3 ICD (B). In HUVEC, <i>DLL1</i> transcripts were detected (D). In HBVP and HUVEC, <i>JAGGED1</i> transcripts were detected (respectively E and F). The presented data are means ± SEM, n = 3 and are representative of at least two independent experiments. n.s., non significant. ***, P<0.0001.</p
