Progress curve analysis of the wild-type and mutant xoFabV variants in the NADH oxidation assay.

Abstract

<p>The enzyme activity of wild-type and mutant xoFabV was determined by monitoring the oxidation of NADH to NAD⁺ at 340 nm. The reaction was initiated by adding the substrate crotonyl-CoA and was monitored for 10 min at 25°C.</p