Redox state and NADH level in fibroblasts with PINK1 mutations and control cells.

Abstract

<p>A–B Graphs demonstrate averaged trace of NADH autofluorescence in control L2132 fibroblasts (A) and L1703 PINK1 mutation (B). Estimation of the %age change in mitochondrial redox level in control cells – L2132 (A) and cells with mutations in PINK1 (B–C). C-Redox state was estimated as: 0 is response to FCCP (maximal rate of respiration and lowest level of mitochondrial NADH) and 100% is response to cyanide (inhibition of respiration with no consumption of NADH in mitochondria; Fibroblasts with PINK1 mutations have lower NADH redox state compared to control fibroblasts (except L2126) that can be normalized by application of 5 mM glutamate (C). D – Total pool of mitochondrial NADH was also significantly lower in fibroblasts with PINK1 mutations.</p