A rise in [Ca²⁺]_c induces mitochondrial depolarisation in fibroblasts with PINK1 mutations.

Abstract

<p>Arrows mark UV-induced flash photolysis of cells loaded with Ca-NP-EGTA, fluo-4 and TMRM. In control C3 (A), L2132 (B) and in fibroblast with L2122 mutation (C) demonstrated an increase in [Ca²⁺]c in response to flash photolysis, with no change in Δψ_m. In fibroblasts with PINK1 mutations (L2123 –D; L2124 – E; L2126 – F; and L1703-G), flash photolysis induced an increase in [Ca²⁺]_c with profound depolarisation of the mitochondria.</p