Immunohistological localisation detects complement factor C9, but not the activated SC5b-9 complex in scabies mite infested human skin sections.

Abstract

<p>Histological sections were probed with antibodies raised against human SC5b-9 neoantigen (2) and against complement factor C9 (6), with anti-human IgG (3 and 7) and the respective pre-immune sera from goat (4) and rabbit (8). Red staining indicates binding of primary antibody. A schematic diagram in the left panel (1 and 5) outlines the features in the histological sections. The mite gut and fecal pellets are shown in red, the mite body in gray, the burrow in white and the epidermis in blue. b, burrow; f, feces; g, gut; m, mite. Scale bars (100 µM) indicate the magnification level.</p