<p>(A) Schematic presentation of GPI-anchored PrP<sup>C</sup>WT and the PrP<sup>C</sup> fusion protein with the GPI-anchor of Thy-1 (PrP<sup>C</sup>-GPIThy-1). The substitution of the GPI-anchor signal sequence (ss) of the PrP for the one of Thy-1 is indicated. (B) Western blots of PrP<sup>C</sup>WT and PrP<sup>C</sup>-GPIThy-1 stably expressed in MDCK cells. A clone with a similar expression level as PrP<sup>C</sup>WT was chosen. The glycotype of di-, mono-, and non-glycosylated PrP<sup>C</sup>-GPIThy-1 is unchanged. (C) Assessment of non-permeabilized membrane localization of PrP<sup>C</sup>WT and PrP<sup>C</sup>-GPIThy-1 by confocal microscopy shows plasma membrane localization of both proteins (scale bar is 10 µm). (D) Sucrose density gradient centrifugation of 1% Triton-X100 extraction at 4°C of PrP<sup>C</sup>WT and PrP<sup>C</sup>-GPIThy-1 cells reveal localization of both in flotillin enriched DRMs. Fractions were taken from the top (fraction 1) to the bottom (fraction 12).</p
