Effect of DEC1 SUMOylation on the repression of CLOCK/BMAL1 activity.

Abstract

<p>(A) MCF-7 cells were transfected with HA-tagged wild-type DEC1 or its mutant 2K/2R with 3xE-box-luciferase reporter and Flag-tagged CLOCK/BMAL1 and luciferase activity was determined. (B) MCF-7 cells were transfected as in (A) but with increasing concentrations of wild-type DEC1 or 2K/2R and luciferase activity was then determined. (C) Expressions of CLOCK and DEC1 in cells from (B) were detected by western blot with anti-Flag or anti-HA antibody. (D) MCF-7 cells were transfected with HA-tagged wild-type DEC1 or 2K/2R together with Myc-tagged HDAC1. Cell lysate was subjected to IP with anti-HA antibody followed by western blot with anti-Myc antibody. *, <i>p</i><0.05; **, <i>p</i><0.01.</p