<p><b>A</b>. qRT-PCR of mRNA isolated from MCF7 cells transfected with plasmids including vector pcDNA-HA (-), pcDNA-HA-NEDD9 (N), pcDNA-HA-BCAR1 (B), or pcDNA-HA-NEDD9 and pcDNA-HA-BCAR1 (NB). <b>B</b>. MCF7 transfected with siRNAs including scrambled control (SCR), or targeting NEDD9 (siN), BCAR1 (siB) and both (siNB), were stained for E-cadherin (red), and DAPI (blue). <b>C</b>. MCF7 cells transfected as in <b>A</b> and stained for E-cadherin (red), HA-fused proteins (green) and DAPI (blue). Arrows indicate cell-cell contacts of transfected cells. <b>D</b>. MCF7 cells transfected with plasmids including vector pcDNA-GFP, pcDNA-GFP-NEDD9, pcDNA-GFP-BCAR1, or pcDNA-GFP-NEDD9 and pcDNA-GFP-BCAR1 (green) were stained for β-catenin (red), and DAPI (blue). Arrowheads point to β-catenin localization at the focal adhesions. Scale bar in <b>B–D</b>, 20 µm. <b>E</b>. Immunofluorescence demonstrating that β-catenin localizes to focal adhesions in MCF7 cells expressing pcDNA-GFP-NEDD9 and pcDNA-GFP-BCAR1 (green). β-catenin (red) and the focal adhesion protein paxillin (blue) are indicated. Scale bar, 20 µm. Bottom panel represents magnifications of indicated areas from boxes.</p
